《植物生理学报》 2009, 45(5): 455-458
通信作者:谭金芳;E-mail: tanjf@henau.edu.cn;Tel: 0371-63554088
摘 要:
研究外源一氧化氮(NO)供体硝普钠(SNP)对干旱胁迫下小麦幼苗叶片ATP酶活性和膜脂过氧化影响的结果表明, 15%聚乙二醇-6000 (PEG-6000)模拟的干旱胁迫下小麦幼苗叶中H+-ATP 酶和Ca2+-ATP 酶活性显著升高后迅速下降, 硫代巴比妥酸反应产物(TBARS)和质量膜透性增加; 0.1 mmol·L-1 SNP可提高干旱胁迫下小麦幼苗叶中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性, 降低超氧阴离子(O2-·)和过氧化氢(H2O2)水平, 缓解膜脂过氧化, 稳定生物膜的结构和功能, H+-ATP 酶和Ca2+-ATP 酶也可以保持更高的活性。关键词:一氧化氮; 干旱胁迫; 小麦幼苗叶片; ATP酶活性; 膜脂过氧化
收稿:2009-01-15 修定:2009-04-20
资助:国际植物营养研究所(IPNI)项目(30200108)、“ 十一五”国家科技支撑计划(30200166)和河南省农业科技成果转化基金项目(05360000006)。
Corresponding author: TAN Jin-Fang; E-mail: tanjf@henau.edu.cn; Tel: 0371-63554088
Abstract:
The effects of nitric oxide (NO) donor sodium nitroprusside (SNP) on ATPase activity and membrane lipid peroxidation in the wheat (Triticum aestivum cv. ‘Luohan 6’) seedling leaves under drought stress were investigated. Results showed that the 15% PEG-6000 treatment apparently enhanced the H +-ATPase andCa2+-ATPase activities before period of deterioration, accelerated the accumulation of thiobarbituric acid reactive substances (TBARS) and membrane permeability in wheat seedling leaves under drought stress. 0.1 mmol·L-1SNP could enhance the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), lower O2¯ production rate, H2O2 content and membrane lipid peroxidation, stabilize the structure and function of biomembranes, increase the activities H+-ATPase and Ca2+-ATPase.Key words: nitric oxide; drought stress; wheat seedling leaves; ATPase activity; membrane lipid peroxidation
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